Ọrịa ọnya afọ ọsịsa nke Porcine (SADS-CoV) bụ ọrịa porcine enteric pathogenic nke achọpụtara ọhụrụ nke nwere ike bute afọ ọsịsa mmiri na ezì amụrụ ọhụrụ ma bute nnukwu mfu akụ na ụba na ụlọ ọrụ ezi.Ka ọ dị ugbu a, ọ nweghị usoro serological dabara adaba iji nyochaa ịdị irè nke ọrịa SADS-CoV na ọgwụ mgbochi, yabụ ọ dị mkpa ngwa ngwa iji ọgwụ mgbochi immunosorbent na-ejikọta enzyme (ELISA) dị irè iji mejupụta ụkọ a.N'ebe a, plasmid recombinant na-egosipụta protein SADS-CoV spike (S) jikọtara ya na ngalaba IgG Fc mmadụ ka arụpụtara iji mepụta baculovirus recombinant wee gosipụta na sel HEK 293F.A na-eji protein G resin sachaa protein S-Fc ma na-ejigide mgbochi mmadụ Fc na mgbochi SADS-CoV.Mgbe ahụ, ejiri protein S-Fc mepụta ELISA na-apụtaghị ìhè (S-iELISA) wee kwalite ọnọdụ mmeghachi omume nke S-iELISA.N'ihi ya, site n'ịtụle uru OD450nm nke 40 SADS-CoV sera na-adịghị mma kwadoro site na immunofluorescence assay (IFA) na Western blotting, ọnụ ahịa mbelata bụ 0.3711.Ọnụọgụ nke mgbanwe (CV) nke 6 SADS-CoV nti sera n'ime na n'etiti ọsọ nke S-iELISA niile erughị 10%.Nnwale nlegharị anya nke cross-reactivity gosiri na S-iELISA enweghị ihe ọ bụla na-emeghachi omume na sera virus porcine ndị ọzọ.Na mgbakwunye, dabere na nchọpụta nke 111 ụlọ ọgwụ serum sample, mkpokọta ndakọrịta ọnụego nke IFA na S-iELISA bụ 97.3%.Nnwale nkwụsị nke nje ahụ na ụkpụrụ 7 dị iche iche OD450nm nke serum gosiri na uru OD450nm nke S-iELISA chọpụtara nwere njikọ chiri anya na nnwale neutralization nje.N'ikpeazụ, a rụrụ S-iELISA na ụdị serum ugbo 300.Ngwa azụmaahịa nke enterovirus porcine ndị ọzọ gosipụtara na ọnụego IgG dị mma nke SADS-CoV, TGEV, PDCoV na PEDV bụ 81.7%, 54%, na 65.3%, n'otu n'otu., 6%, n'otu n'otu.Nsonaazụ na-egosi na S-iELISA bụ kpọmkwem, na-enwe mmetụta, na mmeghari, enwere ike iji ya chọpụta ọrịa SADS-CoV na ụlọ ọrụ ezì.Eji nwebiisinka chedoro edemede a.ikike niile echekwabara.
Oge nzipu: Jun-22-2021